L2 cells were exposed to 2.5% Essential GSH® and
intracellular glutathione was measured after 30 minutes, 1 hour, 2
hours, 3 hours, and 4 hours. Intracellular glutathione content was
measured via the spectrophotometric recycling assay, normalized to
total cellular protein.
Results clearly indicate that
intracellular glutathione levels increased after exposure to
Essential GSH®, peaking at 2 hours with over a 2-fold
increase. The transient action of the increased glutathione
remains unknown, although repression of the endogenous
glutamate-cysteine ligase (GCL) enzyme through GSH-feedback
inhibition may be a likely reason.
Tests performed by:
Dale Dickinson, PhD
Department of Environmental Sciences
University of Alabama at Birmingham